Normal view MARC view ISBD view

Induction Of Ovulatory Oestrus In Non Cycling Buffalo Heifers During Summer

By: Iqbal Rahim | Dr. Kazim Raza Chohan.
Contributor(s): Dr. Muhammad | Dr. Muhammad Aleem | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 1995Subject(s): Department of TheriogenologyDDC classification: 0424,T Dissertation note: Late maturity in buffalo is one of the major problem which limits its overall productive and reproductive performance. Summer anoestrus plays role to delay the onset of puberty. The present study was therefore conducted to induce oestrus in non-cycling buffalo heifers during summer. Eighteen healthy non-cycling buffalo heifers with 431.38±41.5 kg body weight and 1190.44±170.92 days of age were utilized. These animals were randomly divided into three equal groups viz; group A, B and C; each comprising of 6 animals. Heifers in group A were inserted an ear implant containing (3 mg norgestomet) for 9 days and an injection of oestradiol valerate at implant insertion. At implant withdrawal 700 IU of PMSG was administered intramuscularly. Later these animals were inseminated twice at 12 and 20 hours after the onset of oestrus. The heifers in group B were given 2 injections of 500 pg cloprotenol (2 cc estrumate) at 11 days interval and were given fixed time insemination at 72 and 96 hours after second injection. The heifers in group C received no treatment and served as controls. The animals in oestrus from group C were inseminated at 12 and 20 hours after the onset of oestrus. The rectal palpation was performed for ovarian structures twice in a week for two consecutive weeks and at oestrus for follicular development and on day 8 and 10 after oestrus for corpus luteum. Pregnancy was diagnosed on day 60 post insemination. Blood samples were collected on day 8 and 10 after oestrus for progesterone estimation. Progesterone concentration was estimated by enzyme immuno assay technique. All the heifers in group A exhibited oestrus within 49.83 ± 12.65 hours; after PMSG injection. No animal from group B exhibited oestrus whereas only one heifers from control group C exhibited oestrus. The duration of oestrus in buffalo heifers treated with Norgestomet ear implant was 18.00 ± 0.30 hours. The only heifer from group C remained in oestrus for 20 hours. Only one animal (16.66%) conceived at induced oestrus in group A. None of the animal from group B and C became pregnant. Progesterone levels were found to be indicative of ovarian activity in experimental animals. Plasma progesterone concentration of 0.61 ± 0.16 ng/ml was observed on day 8 and 10 in animals showing inovulatory oestrus from group A whereas progesterone values of 5.2 and 0.7 ng/ml were observed in ovulating animals, exhibiting the presence of active corpus luteum. Low progesterone levels of 0.62 ± 0.17 ng/ml were observed in heifers from group B showing the non functional status of the ovarian. Plasma progesterone levels of 5.2 and 7.5 ng/ml was observed on day 8 and 10 after insemination in the only heifers from the control group C. The remaining 5 animals were having smooth ovaries with basal levels of plasma progesterone (0.53_+ 0.9ng/ml). In conculsion Norgestome treatment was found to be effective for induction of oestrus in buffalo heifers during summer. However studies whoud be undertaken to determine the reasons of lower conception rate. Prostaglandin (PGF2a) treatment was found ineffective in noncycling true anoestrus buffalo heifers.
Tags from this library: No tags from this library for this title. Add tag(s)
Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 0424,T (Browse shelf) Available 0424,T
Total holds: 0

Late maturity in buffalo is one of the major problem which limits its overall productive and reproductive performance. Summer anoestrus plays role to delay the onset of puberty. The present study was therefore conducted to induce oestrus in non-cycling buffalo heifers during summer. Eighteen healthy non-cycling buffalo heifers with 431.38±41.5 kg body weight and 1190.44±170.92 days of age were utilized. These animals were randomly divided into three equal groups viz; group A, B and C; each comprising of 6 animals. Heifers in group A were inserted an ear implant containing (3 mg norgestomet) for 9 days and an injection of oestradiol valerate at implant insertion. At implant withdrawal 700 IU of PMSG was administered intramuscularly. Later these animals were inseminated twice at 12 and 20 hours after the onset of oestrus. The heifers in group B were given 2 injections of 500 pg cloprotenol (2 cc estrumate) at 11 days interval and were given fixed time insemination at 72 and 96 hours after second injection. The heifers in group C received no treatment and served as controls. The animals in oestrus from group C were inseminated at 12 and 20 hours after the onset of oestrus. The rectal palpation was performed for ovarian structures twice in a week for two consecutive weeks and at oestrus for follicular development and on day 8 and 10 after oestrus for corpus luteum. Pregnancy was diagnosed on day 60 post insemination. Blood samples were collected on day 8 and 10 after oestrus for progesterone estimation. Progesterone concentration was estimated by enzyme immuno assay technique. All the heifers in group A exhibited oestrus within 49.83 ± 12.65 hours; after PMSG injection. No animal from group B exhibited oestrus whereas only one heifers from control group C exhibited oestrus. The duration of oestrus in buffalo heifers treated with Norgestomet ear implant was 18.00 ± 0.30 hours. The only heifer from group C remained in oestrus for 20 hours. Only one animal (16.66%) conceived at induced oestrus in group A. None of the animal from group B and C became pregnant. Progesterone levels were found to be indicative of ovarian activity in experimental animals. Plasma progesterone concentration of 0.61 ± 0.16 ng/ml was observed on day 8 and 10 in animals showing inovulatory oestrus from group A whereas progesterone values of 5.2 and 0.7 ng/ml were observed in ovulating animals, exhibiting the presence of active corpus luteum. Low progesterone levels of 0.62 ± 0.17 ng/ml were observed in heifers from group B showing the non functional status of the ovarian. Plasma progesterone levels of 5.2 and 7.5 ng/ml was observed on day 8 and 10 after insemination in the only heifers from the control group C. The remaining 5 animals were having smooth ovaries with basal levels of plasma progesterone (0.53_+ 0.9ng/ml). In conculsion Norgestome treatment was found to be effective for induction of oestrus in buffalo heifers during summer. However studies whoud be undertaken to determine the reasons of lower conception rate. Prostaglandin (PGF2a) treatment was found ineffective in noncycling true anoestrus buffalo heifers.

There are no comments for this item.

Log in to your account to post a comment.


Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.